Highly efficient bi-allelic mutation rates using TALENs in Xenopus tropicalis

In the past decade, Xenopus tropicalis has emerged as a powerful new amphibian genetic model system, which offers all of the experimental advantages of its larger cousin, Xenopus laevis. Here we investigated the efficiency of transcription activator-like effector nucleases (TALENs) for generating targeted mutations in endogenous genes in X. tropicalis. For our analysis we targeted the tyrosinase (oculocutaneous albinism IA) (tyr) gene, which is required for the production of skin pigments, such as melanin. We injected mRNA encoding TALENs targeting the first exon of the tyr gene into two-cell-stage embryos. Surprisingly, we found that over 90% of the founder animals developed either partial or full albinism, suggesting that the TALENs induced bi-allelic mutations in the tyr gene at very high frequency in the F0 animals. Furthermore, mutations tyr gene were efficiently transmitted into the F1 progeny, as evidenced by the generation of albino offspring. These findings have far reaching implications in our quest to develop efficient reverse genetic approaches in this emerging amphibian model

Estudio de la Flora Vascular de Ilundáin (Navarra).

En este trabajo hemos realizado un estudio de la Flora Vascular presente en Ilundáin (Navarra) en una superficie aproximada de 1.35 km2. Para ello se han recolectado los ejemplares presentes en la zona durante un periodo de tres años (de 1998 a 2001) mediante la realización de herborizaciones de los distintos ambientes, diferenciados por la vegetación dominante de cada uno (robledal, hayedo, pinar, matorral,...) y en las distintas estaciones del año. Así se han recolectado 730 ejemplares pertenecientes a 294 taxones diferentes. Se ha creado un Catálogo Florístico de la zona bajo estudio donde cada taxon está acompañado por información referente a la nomenclatura (sinónimos y nombres vulgares), información biogeográfica (tipo corológico) e información ecológicofuncional (hábitats o ambientes en los que aparece, especies acompañantes, fenología, tipo biológico,...) así como todas las citas tomadas de cada uno de ellos

Metabolomics profiling of strawberry (Fragaria x ananassa) F1 population to characterize flavour and nutritional traits

The cultivated strawberry (Fragaria x ananassa) is a highly consumed fruit known for its delicate flavour and nutritional characteristics. However, as fruit quality attributes have been lost after years of traditional breeding, new technological tools, such as high throughput metabolomics, are necessary for the identification of factors responsible of these traits. Here we present the metabolomics profiling for the content of primary and secondary metabolites of a 95 F1 individuals strawberry population derived from genotype “1392”, selected for its superior flavour, and “232” (Zorrilla-Fontanesi et al., 2011; Zorrilla-Fontanesi et al., 2012). Metabolite profiling was performed on mature fruits of the strawberry population using gas chromatography hyphenated to time-of-flight mass spectrometry for primary metabolites and ultra performance liquid chromatography Exactive Orbitrap tandem mass spectrometry for secondary metabolites.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Use of the Ames Check Standard Model for the Validation of Wall Interference Corrections

The new check standard model of the NASA Ames 11-ft Transonic Wind Tunnel was chosen for a future validation of the facility's wall interference correction system. The chosen validation approach takes advantage of the fact that test conditions experienced by a large model in the slotted part of the tunnel's test section will change significantly if a subset of the slots is temporarily sealed. Therefore, the model's aerodynamic coefficients have to be recorded, corrected, and compared for two different test section configurations in order to perform the validation. Test section configurations with highly accurate Mach number and dynamic pressure calibrations were selected for the validation. First, the model is tested with all test section slots in open configuration while keeping the model's center of rotation on the tunnel centerline. In the next step, slots on the test section floor are sealed and the model is moved to a new center of rotation that is 33 inches below the tunnel centerline. Then, the original angle of attack sweeps are repeated. Afterwards, wall interference corrections are applied to both test data sets and response surface models of the resulting aerodynamic coefficients in interference-free flow are generated. Finally, the response surface models are used to predict the aerodynamic coefficients for a family of angles of attack while keeping dynamic pressure, Mach number, and Reynolds number constant. The validation is considered successful if the corrected aerodynamic coefficients obtained from the related response surface model pair show good agreement. Residual differences between the corrected coefficient sets will be analyzed as well because they are an indicator of the overall accuracy of the facility's wall interference correction process

Critical properties and Bose Einstein Condensation in dimer spin systems

We analyze the spin relaxation time $1/T_1$ for a system made of weakly coupled one dimensional ladders.This system allows to probe the dimensional crossover between a Luttinger liquid and a Bose-Einstein condensateof magnons. We obtain the temperature dependence of $1/T_1$ in the various dimensional regimes, and discuss the experimental consequences.Comment: 4 pages, RevTeX 4, 3 EPS figure

Classical simple Lie 2-algebras of odd toral rank and a contragredient Lie 2-algebra of toral rank 4

After the classification of simple Lie algebras over a field of characteristic p > 3, the main problem not yet solved in the theory of finite dimensional Lie algebras is the classification of simple Lie algebras over a field of characteristic 2. The first result for this classification problem ensures that all finite dimensional Lie algebras of absolute toral rank 1 over an algebraically closed field of characteristic 2 are soluble. Describing simple Lie algebras (respectively, Lie 2-algebras) of finite dimension of absolute toral rank (respectively, toral rank) 3 over an algebraically closed field of characteristic 2 is still an open problem. In this paper we show that there are no classical type simple Lie 2-algebras with toral rank odd and furthermore that the simple contragredient Lie 2-algebra G(F4,a) of dimension 34 has toral rank 4. Additionally, we give the Cartan decomposition of G(F4,a)

Effect of light and temperature on carotenogenesis by rhodotorula mucilaginosa.

The present work had the objective of studying the effect of temperature and light on the production of carotenoids by wild Rhodotorula mucilaginosa, isolated in Brazil, using central composite design (CCD)

Experimental demonstration of gridless spectrum and time optical switching

An experimental demonstration of gridless spectrum and time switching is presented. We propose and demonstrate a bit-rate and modulation-format independent optical cross-connect architecture, based on gridless spectrum selective switch, 20-ms 3D-MEMS and 10-ns PLZT optical switches, that supports arbitrary spectrum allocation and transparent time multiplexing. The architecture is implemented in a four-node field-fiber-linked testbed to transport continuous RZ and NRZ data channels at 12.5, 42.7 and 170.8 Gb/s, and selectively groom sub-wavelength RZ channels at 42.7 Gb/s. We also showed that the architecture is dynamic and can be reconfigured to meet the routing requirements of the network traffic. Results show error-free operation with an end-to-end power penalty between 0.8 dB and 5 dB for all continuous and sub-wavelength channels

Pressure-induced Superconductivity in a Ferromagnet UGe2_2 -- Resistivity Measurements in Magnetic Field --

The electrical resistivity measurements in the magnetic field are carried out on the pressure-induced superconductor UGe$_2$. The superconductivity is observed from 1.06 to 1.44 GPa. The upper critical field of $H_{C2}$ is anisotropic where $H_{C2}(T)$ exhibits positive curvature for $H//b$ and $c$-axis. The characteristic enhancement of $H_{C2}$ is reconfirmed for $H//a$-axis. In the temperature and field dependence of resistivity at $P > P_{C}$ where the ferromagnetic ordering disappears, it is observed that the application of the external field along the {\it a}-axis increases the coefficient of Fermi liquid behavior $AT^{2}$ correspondingly to the metamagnetic transition.Comment: To be published in the proceeding of the International Conference on High Pressure Science and Technology(AIRAPT-18),Beijing,China,23-27 July 200